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Plasmodium Aldolase In Malaria Test

Posted by anna on March 17, 2022 

One of the new methods for detecting Plasmodium aldolase antibody is microfluidic chip. The technology utilizes microfluidic channels that direct flow without mechanical or electric valves. Burst valves release reagents based on centrifugal force. Capillary burst valves have no moving parts and rely on surface tension to keep fluid in place. They are especially useful for rapid detection.

There are dozens of potential biomarkers of Plasmodium parasites. The enzyme, which is involved in the breakdown of glycogen, has been identified as a promising candidate. Although aldolase is present in most animal species, the human-specific enzyme is antigenically distinct. It is found on the membrane of infected red blood cells. It is important to note that the presence of pLDH in a serum sample indicates a high probability of malaria infection.

Researchers have previously isolated monoclonal antibodies against Plasmodium aldolase that recognize the 41-kDa protein band. This antigen is also used to detect P. falciparum infection in monkeys. This study has shown that the antibodies produced against aldolases from different species cross-react with each other, indicating a high degree of conservation. During evolution, the enzyme was discovered to be present in the cytoplasm of infected red blood cells. However, the inactive form of the enzyme is associated with a membrane fraction. Furthermore, the digestion data suggest that the membrane-associated enzyme forms an oligosaccharide anchor.

Plasmodium aldolase is found in all animal species. It is important for the intraerythrocytic merozoite life stage. It binds to Actin, Adhesin, and Thrombospondin-related anonymous protein. The amino acid sequence of the enzyme is highly conserved, and the protein can be used as a diagnostic marker for malaria.

The antibodies against Plasmodium aldolase are pan-specific and have been used in an immunochromatographic test. They are effective for the diagnosis of malaria. The enzyme is found in the cytoplasm of the parasites and is located in the cellular membrane. Both forms of the enzyme can be detected by different methods. For example, infected red blood cells contain high levels of the enzyme.

There are dozens of potential biomarkers for malaria. The Plasmodium aldolase is one of the most widely studied. It is found on red blood cells and is critical for intraerythrocytic merozoite development. The enzyme is also a useful diagnostic marker. It is found in feces and is antigenically dissimilar to human and animal species. The enzyme is a pan-specific protein that binds to the actin filaments of infected merozoites.

Other tests for Plasmodium aldolase include the histidine-rich protein (HRP) 2 and Pf-LDH. The sensitivity of these antibodies varies with different malaria species, but the Pf-LDH sensitivity was greater than that of pHRP2. While these techniques may not be completely effective for all cases, they can help the doctor diagnose a patient's parasite infection.

Plasmodium Aldolase Multiplex ELISA

Plasmodium aldolase is an intracellular enzyme that catalyzes the oxidation of lactate to pyruvate. The enzyme binds to nicotinamide adenine dinucleotide (NAD), which is a co-enzyme. As a target, this antigen is a promising drug candidate. Other uses for this protein include coupling the actomyosin motor and affecting cell motility and invasion by the host.

A variety of Plasmodium antigens are used to detect the presence of the parasite. In a sandwich ELISA, a capture antibody is added to the test sample. The secondary antibody is conjugated to horse radish peroxidase and is purchased from a commercial source. The assay buffers are Tween-20 and bovine serum albumin (5%) are used for blocking.

A microfluidic chip can detect Plasmodium aldolase in the bloodstream and deliver antibodies to a reaction chamber. Microfluidic channels are designed to direct flow without using mechanical or electrical valves. The capillary burst valve is a passive reagent release system and does not require any moving parts. Surface tension holds the fluid in place until it reaches the rotational speed of the bead.

The multiplex ELISA technique is highly sensitive and specific. Several antibodies have been developed to detect aldolase in a variety of species. The pan-Platomodium aldolase and P. vivax LDH antibodies were purchased from Luminex. The two primary antibodies were then conjugated to different regions of the bead using a Luminex antibody coupling kit. A single bead was loaded with 12.5 ug of anti-PvLDH and a second bead with horse radish peroxidase.

The pLDH bead typically gives low signals for the two species. The aldolase-pLDH assay has been shown to detect aldolase at lower concentrations than pLDH. This method has several advantages. For example, it has a higher sensitivity than the other. In addition, the assay is fast and can be used for screening and diagnosis of malaria.

The recombinant phage display library is a highly sensitive and specific antibody that targets both P. falciparum and P. vivax. It also shows high sensitivity in patients with malaria, although the test is still not 100% accurate. The results of this assay depend on the patient's condition and the type of parasites. There are two main strains of Plasmodium: the recombinant and the non-recombinant.

These monoclonal antibodies are pan-specific and detect both pLDH and HRP-2. They are used in combination with other tests to differentiate the four species of Plasmodium. They are also sensitive to detect the asexual stages of the parasite. This antibody is not specific to the parasite species. It is useful for detecting the presence of the parasites during pregnancy, but it cannot be used to differentiate them.

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